Case Study
Enterovirus 71 is a virus of the genus Enterovirus in the Picornaviridae family notable for its etiological role in epidemics of severe neurological diseases in children. It was first isolated and characterized from cases of neurological disease in California in 1969. Enterovirus 71 infrequently causes polio-like syndrome permanent paralysis. (cited from wikipedia)
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No.
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Process Character
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How TideCell supports the process requirement?
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Can other systems do the same?
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1.
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High cell density is required to achieve high titer, and reduce medium consumption.
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High surface area provided in TideCell increase cell density up to 10 folds and save culture medium up to 90%.
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Roller bottle
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No. low cell density due to limitation of surface area
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Microcarrier stir tank
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No. the bead density is limited
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Fixed bed bioreactor,
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Yes, but the scale is limited.
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hollow fiber bioreactor
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Yes, but the scale is limited.
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2.
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Cells tend to detach during post-infection period.
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A gentle and relative static culture method enables a low shear stress culture environment. Cells will be not easy to detach after infection and increase the productivity.
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Roller bottles
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Yes. Shear stress is low.
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Microcarrier stir tank
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No. Shear stress is higher. Cells tend to detach during post-infection period
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Fixed bed bioreactor
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No. Shear stress is higher. Cells tend to detach during post-infection period
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3.
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Infection time is crucial.
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Easy to take carrier sample for cell counting and MOI calculation.
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Roller bottle
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Yes. Can decide the infection time by observing the confluency from the bottles. But no exact cell density. MOI calcuation is rough.
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Microcarrier stir tank
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Yes. Carrier sample can be taken for cell counting.
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Fixed bed bioreactor
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No. Indirect estimation by DO or Glucose consumption rate. Difficult to calculate MOI.
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4.
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Require to adjust matrix to culture medium ratio
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Roller bottle
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Microcarrier stir tank
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Fixed bed bioreactor
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5.
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Require to reduce impurities to increase the recovery rate.
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Roller bottle
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Microcarrier stir tank
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Fixed bed bioreactor
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6.
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Final Achievement
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TCID50: log 7.5
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Roller bottle
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Microcarrier stir tank
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Log 7.0
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